"

 

From 2008.igem.org

In the exponential phase of growth, at the steady state, we have

so, with

that gives, after reducing the expression

with the previous expression of [TetR]_eq

so, finally, if we use a calculated value of [TetR] (by using the expression of ƒ0 for instance, or by introducing a value given by tetR after an other "caracterised promoter")

param	signification	unit	value
[expr(pTet)]	expression rate of pTet with RBS E0032	nM.min-1	see "findparam" need for 20 measures
γGFP	dilution-degradation rate of GFP(mut3b)	min-1	0.0198
[GFP]	GFP concentration at steady-state	nM	need for 20 measures
(fluorescence)	value of the observed fluorescence	au	need for 20 measures
conversion	conversion ration between fluorescence and concentration	nM.au-1	(1/79.429)

param	signification corresponding parameters in the equations	unit	value
βtet	production rate of pTet with RBS E0032 β1	nM.min-1
(Ktet/{coeftetR}ntet)	activation constant of pTet K18	nM
ntet	complexation order of pTet n18	no dimension
KaTc	complexation constant aTc-TetR K17	nM
naTc	complexation order aTc-TetR n17	no dimension

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers