"
Team:Paris/Modeling/f8
From 2008.igem.org
and
so the expression
gives
and
and for calculated values of the TF,
and
| param | signification | unit | value |
| [expr(pFlhB)] | expression rate of pFlhB with RBS E0032 | nM.s-1 | see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM? |
| γGFP | dilution-degradation rate of GFP(mut3b) | s-1 | ln(2)/3600 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 + 20 measures and 5x5 measures for the SUM? |
| (fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the SUM? |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| β6 | production rate of FlhDC-pFlhB with RBS E0032 β6 | nM.s-1 | |
| (K4/{coefflhDC}n4) | activation constant of FlhDC-pFlhB K4 | nMn4 | |
| n4 | complexation order of FlhDC-pFlhB n4 | no dimension | |
| β14 | production rate of FliA-pFlhB with RBS E0032 β14 | nM.s-1 | |
| (K10/{coeffliA}n10) | activation constant of FliA-pFlhB K10 | nMn10 | |
| n10 | complexation order of FliA-pFlhB n10 | no dimension |
Then, if we have time, we want to verify the expected relation
