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Team:Illinois/Protocols
From 2008.igem.org
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Current Tasks and Subgroup Status
Yeast Culture
[http://www.protocol-online.org/prot/Model_Organisms/Yeast/Yeast_Culture/ Longterm storage and streaking protocols]
[http://www.mrw.interscience.wiley.com.proxy2.library.uiuc.edu/emrw/9780471142720/cp/cpmb/article/mb1302/current/pdf Growth and manipulation of yeast]
[http://openwetware.org/wiki/Yeast_culture_media Yeast culture media]
[http://www.protocol-online.org/prot/Model_Organisms/Yeast/Yeast_Transformation/ Yeast transformation protocols]
Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual (1998 edition available in Biology library, newer editions currently checked out)
[http://openwetware.org/wiki/Yeast Yeast Info on OWW] Contains General, Protocols, Materials, and References Info - Alzar
Directed Evolution
Malaria
The malaria group has found that malaria would be a poor target as the protazoan is never found in the water during its lifecycle. Our new target is typhoid or cholera.
E. coli
check e.coli research articles
http://cgsc.biology.yale.edu/
[http://www.ciwemb.edu/labs/koshland/Protocols/BACTERIA/ecolicells.html Frozen Competent E.Coli Cells. Methods and protocol for freezing E.Coli Bacteria Competent Cells in aliquots for later use re-use.]
[http://minds.wisconsin.edu/handle/1793/7956 Opimization of recombitant E. coli protein expression in growth medium]
[http://books.google.com/books?id=TH7OJGY7Br8C&pg=PA1&dq=protein+expression+e+coli&as_brr=0&sig=S18cODgXA7zmQFbsVcrOBwPVKRU#PPA92,M1 E. coli gene expression protocols (book available online on google books)]
[http://www.pepcore.embl.de/protein_expression/ecoli/index.html General indications and protocols for the expression in bacteria]
[http://forums.biotechinstitute.org/showthread.php?t=60 Glycerol storage of E. coli bacterial stocks]
[http://www.teknova.com/liquids/liquid-tbs.shtml Different liquid culture media for E. coli]
[http://www.teknova.com/dry_media/dry-lbagars.shtml Different dry culture media for bacterial growth]
[http://www.creatvmicrotech.com/Biosensor-journal%20papers/1.paper_15-2005-Zhu-BB1.pdf Detection of water-borne E. coli O157 using the integrating waveguide biosensor]
GPCRs
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