"
User:University of Washington/28 August 2008
From 2008.igem.org
LuxR from AraC and TetR(Faifan)
- ligation
- mix
| dH20(ul) | Buffer(ul) | promoter(ul) | GFP(ul) | LuxR(ul) | T4 ligase(ul) | |
|---|---|---|---|---|---|---|
| LuxR | 12.34 | 2 | 1.54 | - | 3.12 | 1 |
| GFP | 13.84 | 2 | 1.54 | 1.62 | - | 1 |
| no insert | 15.46 | 2 | 1.54 | - | - | 1 |
| no T4 ligase | 14.84 | 2 | 1.54 | 1.62 | - | - |
- incubate at room temp 45 mins
- denature at 65 degree Celsius 10 mins
- filtered 20 mins
- transformed 2ul DNA into 45 ul XL1-Blue
- inoculated on CAM plates
Back to Team:University_of_Washington/Notebook#Notebook
