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Team:University of Lethbridge/Notebook/Project2September
From 2008.igem.org
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September 1, 2008
Roxanne
-Picked the colonies which appear to have amplified during the screening PCR, and Incubated them in LB+amp media at 37.0C overnight.
September 2, 2008
Roxanne
-Plasmid Prepped the colonies which were incubated overnight. Ran Plasmids on a 1% Agarose Gel at 100V for 27 minutes.
Nathan Puhl
-Visulaized the Gel, and chose appropriate cells to glycerol stock.
September 3, 2008
Roxanne, Nathan Puhl
-Performed a Restriction Digest on RS1 and RS2 from pGEM T-easy using XbaI and SpeI. Ran overnight at 37.0C
Nathan
-Reran the PCR of the riboswtich from the primers and has been working on the Riboswitch throughout the month.
Gel of RS_cheZ and RS1, RS2
Gel of the New Riboswitch PCR product
