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Template:Team:UC Berkeley/Notebook/BX construction
From 2008.igem.org
PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112000
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bx1 Forward Biobricking of {T4 Holin!}
CCATAGAATTCatgAGATCTatggcagcacctagaatatcat
bx2 Reverse Biobricking of {T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct
PCR bx3/bx2 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112001
----------------------------------------
bx3 Forward biobricking of {<T4 Holin!}
CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg
bx2 Reverse biobricking of {<T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattc
PCR bx1/bx4 on Bjh1301 (685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112002
----------------------------------------
bx1 Forward biobricking of {T4 Holin>}
CCATAGAATTCatgAGATCTatggcagcacctagaatatcat
bx4 Reverse biobricking of {T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc
PCR bx3/bx4 on Bjh1301 (682 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112003
----------------------------------------
bx3 Forward Biobricking of {<T4 Holin>}
CCATAGAATTCatgAGATCTgcagcacctagaatatcattttcg
bx4 Reverse Biobricking of {<T4 Holin>}
cgttaGGATCCtttagcccttcctaatattctggcc
PCR bx3/bx4 on Bjh1301 (692 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112004
----------------------------------------
bx5 Forward Biobricking of {a~T4 Holin!}
CCATAGAATTCatgAGATCTgtctatggcagcacctagaat
bx2 Reverse Biobricking of {a~T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct
PCR bx6/bx2 on Bjh1301 (704 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112005
----------------------------------------
bx6 Forward Biobricking of {rbs.T4 Holin!}
CCATAGAATTCatgAGATCTcttaaaaggagggtctatggcagcacctagaatatc
bx2 Reverse Biobricking of {rbs.T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattct
PCR bx7/bx8 on Bjh1303 (325 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112006
----------------------------------------
bx7 Forward biobricking of {T4 Antiholin!}
CCATAGAATTCatgAGATCTatggccttaaaagcaacagcac
bx8 Reverse biobricking of {T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatg
PCR bx9/bx8 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112007
----------------------------------------
bx9 Forward biobricking of {<T4 Antiholin!}
CCATAGAATTCatgAGATCTgccttaaaagcaacagcactt
bx8 Reverse biobricking of {<T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatg
PCR bx7/bx10 on Bjh1303 (322 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112008
----------------------------------------
bx7 Forward biobricking of {T4 Antiholin>}
CCATAGAATTCatgAGATCTatggccttaaaagcaacagcac
bx10 Reverse biobricking of {T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc
PCR bx9/bx10 on Bjh1303 (319 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112009
----------------------------------------
bx9 Forward biobricking of {<T4 Antiholin>}
CCATAGAATTCatgAGATCTgccttaaaagcaacagcactt
bx10 Reverse biobricking of {<T4 Antiholin>}
cgttaGGATCCttcagtctccaatttaatgttc
PCR bx9/bx10 on Bjh1303 (329 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112010
----------------------------------------
bx11 Forward biobricking of {a~T4 Antiholin!}
CCATAGAATTCatgAGATCTctttatggccttaaaagcaacagc
bx8 Reverse biobricking of {a~T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatg
PCR bx12/bx8 on Bjh1303 (339 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112011
----------------------------------------
bx12 Forward biobricking of {rbs.T4 Antiholin!}
CCATAGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc
bx8 Reverse biobricking of {rbs.T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatg
PCR bx13/bx14 on Bjh1302 (526 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112012
----------------------------------------
bx13 Forward biobricking of {T4 Endolysin!}
CCATAGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx14 Reverse biobricking of {T4 Endolysin!}
cgttaGGATCCttatagatttttatacgcgtccc
PCR bx15/bx14 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112013
----------------------------------------
bx15 Forward biobricking of {<T4 Endolysin!}
CCATAGAATTCatgAGATCTaatatatttgaaatgttacgtatag
bx14 Reverse biobricking of {<T4 Endolysin!}
cgttaGGATCCttatagatttttatacgcgtccc
PCR bx13/bx16 on Bjh1302 (523 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112014
----------------------------------------
bx13 Forward biobricking of {T4 Endolysin>}
CCATAGAATTCatgAGATCTatgaatatatttgaaatgttacg
bx16 Reverse biobricking of {T4 Endolysin>}
cgttaGGATCCtagatttttatacgcgtcccaagtg
PCR bx15/bx16 on Bjh1302 (520 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112015
----------------------------------------
bx15 Forward biobricking of {<T4 Endolysin>}
CCATAGAATTCatgAGATCTaatatatttgaaatgttacgtatag
bx16 Reverse biobricking of {<T4 Endolysin>}
cgttaGGATCCtagatttttatacgcgtcccaagtg
PCR bx17/bx16 on Bjh1302 (530 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112016
----------------------------------------
bx17 Forward biobricking of {a~T4 Endolysin!}
CCATAGAATTCatgAGATCTtattatgaatatatttgaaatg
bx16 Reverse biobricking of {a~T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtg
PCR bx18/bx16 on Bjh1302 (542 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112017
----------------------------------------
bx18 Forward biobricking of {rbs.T4 Endolysin!}
CCATAGAATTCatgAGATCTatacttaggaggtattatgaatatatttgaaatgttac
bx16 Reverse biobricking of {rbs.T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtg
