Template:Team:UC Berkeley/Notebook/AL construction

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--Aronlau 01:15, 5 June 2008 (UTC)

PCR al0001/al0002 on pSB3C6-Bjh1501     (508bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112300
-----------------------------------------------
al0001	Forward Biobricking of lambda lysozyme with start and stop codons
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of lambda lysozyme with start and stop codons
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

PCR al0003/al0002 on pSB3C6-Bjh1501     (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112301
-----------------------------------------------
al0003	Forward Biobricking of Lambda Lysozyme with stop codon and no start codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of Lambda Lysozyme with stop codon and no start codon
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

PCR al0001/al0004 on pSB3C6-Bjh1501     (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112302
-----------------------------------------------
al0001	Forward Biobricking of lambda lysozyme with start codon and no stop codon
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0004	Reverse Biobricking of lambda lysozyme with start codon and no stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc

PCR al0003/al0004 on pSB3C6-Bjh1501     (502bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112303
-----------------------------------------------
al0003	Forward Biobricking of lambda lysozyme with no start/stop codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0004	Reverse Biobricking of lambda lysozyme with no start/stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc

PCR al0005/al0002 on pSB3C6-Bjh1501     (512bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112304
-----------------------------------------------
al0005	Forward Biobricking of lambda lysozyme with spacer
CCATAGAATTCatgAGATCTgaagatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of lambda lysozyme with spacer
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

PCR al0006/al0002 on pSB3C6-Bjh1501     (527bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112305
-----------------------------------------------
al0006	Forward Biobricking of lambda lysozyme with rbs site
CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg
al0002	Reverse Biobricking of lambda lysozyme with rbs site
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

PCR al0007/al0008 on pSB3C6-Bjh1502     (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112306
-----------------------------------------------
al0007	Forward Biobricking of lambda holin with start and stop codons
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
al0008	Reverse Biobricking of lambda holin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc

PCR al0009/al0008 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112307
-----------------------------------------------
al0009	Forward Biobricking of lambda holin with stop codon and no start codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0008	Reverse Biobricking of lambda holin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc

PCR al0007/al0010 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112308
-----------------------------------------------
al0007	Forward Biobricking of lambda holin with start and no stop codon
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
al0010	Reverse Biobricking of lambda holin wtih start and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

PCR al0009/al0010 on pSB3C6-Bjh1502     (343bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112309
-----------------------------------------------
al0009	Forward Biobricking of lambda holin with no start and stop codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0010	Reverse Biobricking of lambda holin with no start and stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

PCR al0011/al0008 on pSB3C6-Bjh1502     (353bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112310
-----------------------------------------------
al0011	Forward Biobricking of lambda holin with spacer
CCATAGAATTCatgAGATCTgaagatgccagaaaaacatgacctg
al0008	Reverse Biobricking of lambda holin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc

PCR al0012/al0008 on pSB3C6-Bjh1502     (369bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112311
-----------------------------------------------
al0012	Forward Biobricking of lambda holin with rbs site
CCATAGAATTCatgAGATCTattgggggtaagacCtgaagatgccag
al0008	Reverse Biobricking of lambda holin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc

PCR al0013/al0008 on pSB3C6-Bjh1503     (355bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112312
-----------------------------------------------
al0013	Forward Biobricking of lambda antiholin with start and stop codons
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0008	Reverse Biobricking of lambda antiholin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc

PCR al0014/al0008 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112313
-----------------------------------------------
al0014	Forward Biobricking of lambda antiholin with stop codon and no start codon
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0008	Reverse Biobricking of lambda antiholin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc

PCR al0013/al0010 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112314
-----------------------------------------------
al0013	Forward Biobricking of lambda antiholin with start codon and no stop codon
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0010	Reverse Biobricking of lambda antiholin with start codon and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

PCR al0014/al0010 on pSB3C6-Bjh1503     (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112315
-----------------------------------------------
al0014	Forward Biobricking of lambda antiholin with no start and stop codons
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0010	Reverse Biobricking of lambda antiholin with no start and stop codons
CGttaGGATCCttgatttctaccatcttctactccggc

PCR al0015/al0008 on pSB3C6-Bjh1503     (359bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112316
-----------------------------------------------
al0015	Forward Biobricking of lambda antiholin with spacer
CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc
al0008	Reverse Biobricking of lambda antiholin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc

PCR al0016/al0008 on pSB3C6-Bjh1503     (375bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112317
-----------------------------------------------
al0016	Forward Biobricking of lambda antiholin with rbs site
CCATAGAATTCatgAGATCTccccttattgggggtaagacatgaagCtgccagaaaaacatgacc
al0008	Reverse Biobricking of lambda antiholin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc

Wobble PCR al0017/al0018      (90bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112318
-----------------------------------------------
al0017	Forward Biobricking of bolA promoter
CCATAGAATTCatgAGATCTCCTAAATATTTGTTGTTAAGCTGCAATGGAAACGG
al0018	Reverse Biobricking of bolA promoter
CGttaGGATCCCCCTTTAAATACTAGCCGCTTTTACCGTTTCCATTGCAGCTTAA

Wobble PCR al0019/al0020      (90bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112319
-----------------------------------------------
al0019	Forward Biobricking of mcb promoter
CCATAGAATTCatgAGATCTCAAATTTAGTTCAAATTATCATTGCAAAATATAGT
al0020	Reverse Biobricking of mcb promoter
CGttaGGATCCAACACTAGTTACTTGCCGTAATTAACTATATTTTGCAATGATAA

Wobble PCR al0021/al0022      (92bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112320
-----------------------------------------------
al0021	Forward Biobricking of ftsQ promoter
CCATAGAATTCatgAGATCTaatacctcaccgggtatgaccagccacagcctggtg
al0022	Reverse Biobricking of ftsQ promoter
CGttaGGATCCtacctgcctgacgtgccgccatcggcaccaggctgtggctggtca

Wobble PCR al0023/al0024      (88bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112321
-----------------------------------------------
al0023	Forward Biobricking of ftsAZ promoter
CCATAGAATTCatgAGATCTAAACGGATTAGCTACGTTGATTTGCGTTATGACT
al0024	Reverse Biobricking of ftsAZ promoter
CGttaGGATCCCGCCCAGCCTACTGCCGCTCCAGAGTCATAACGCAAATCAACG