Rensselaer/8 September 2008

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DNA purification digest

Fe(13ug) Plasmid(11ug)

DNA 280ul 95ul

XbaI 10 10

SprI 20 20

Buffer2 40 17.5

BSA 4 1.75

dH2O 46 30.75

____ _____

400 ul 175 ul

Insert checks digest

Fe-mRFP (~1ug)

DNA(30ug/ul) 33

MsCI(3U/ul) 6.7

EcoRI(2U/ul) 1

EcoBuff 5

BSA 0.5

dH2O 3.8

_____

50 ul

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