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Team:Warsaw/Calendar-Main/29 May 2008
From 2008.igem.org
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1. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB 2. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose 3. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB 4. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1mM IPTG 5. Wait for 5 hours 6. OD measurment 7. 1 ml (2x) from p.1, p.2, p.3, p.4 (LB + rifampicin)
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