Optimisation of primers from 19.06
1. PCR on pBD (jaki numerek?) plasmid with OmpaL_N and OmpaP_link primers (annealing length 45s)
2. PCR on pUC19 plasmid with AlphaL_link and AlphaP_XB primers (annealing length 45s)
3. PCR on pUC19 plasmid with OmegaL_link and OmegaP_EPB primers (annealing length 30s)
Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees