"
Page
Discussion
View source
History
teams
Log in
Team:NTU-Singapore/Notebook/25 September 2008
From 2008.igem.org
Revision as of 13:11, 25 October 2008 by
Lalala8585
(
Talk
|
contribs
)
(
diff
)
← Older revision
|
Latest revision
(
diff
) |
Newer revision →
(
diff
)
NTU@iGEM
Project
Introduction
Methodology
Team
Introduction
Team background
Notebook
Dry Lab
Introduction
ODEs used
Modeling Constructs
Parameters used
Deterministic Modeling
Sensitivity Analysis
Stochastic Modeling
Wetlab
Introduction
Materials and Equipment
Protocols
Verification of Detection & Lysis System
Our New Parts
Characterization
Introduction
Standard Promoters
Characterization of pLacI-GFP
pLacI-GFP images and clips
Characterization of pLsrA-YFP
New characterization Proposal
Parameter Estimation & Correlations
Acknowledgements
Thursday 25 September
Minipreps for LsrA-YFP, followed by digestional gel check.
Result: correct bands: 1kb insert (LsrA-RBS with YFP generator); 2kb vector
Make LuxS mutants more competent. Then transform detection system and production system into these newly made LuxS mutants.
Grow W3110 in LB plus 0.8% glucose for 4 hours, centrifuge and take the supernatant (which contains AI-2), store at -20 degrees C.
"
