Team:Warsaw/Calendar-Main/2 June 2008

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Piotr: Transformation of E.coli TOP10 with pZC320.

Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG

PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase

Primers: AIDlNrH and T7pXbSal

Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)

Annealing temperature: 55°C

Time: 4 minutes

Number of cycles: 20




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