"
Team:Warsaw/Calendar-Main/29 May 2008
From 2008.igem.org
Revision as of 13:32, 1 October 2008 by MKrzyszton (Talk | contribs)
 |
|
||||||||
 |
|
||||||||
Piotr: 2. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose. 3. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB. 4. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1mM IPTG. 5. Wait for 5 hours. 6. OD measurement. 7. 1 ml (2x) from p.1, p.2, p.3, p.4 (LB + rifampicin).
|
|
