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Team:NTU-Singapore/Notebook/3 June 2008
From 2008.igem.org
Tuesday 3 June
- Chin Chong (~~)
- Confirmed the orders for the oligo/primers designs to be purchase from 1st Base
- List of things to purchase modified
- Updated time-line for ordering chemicals and consumables
- Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
- ZhenFu
- Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
- Modified model to due to more estimation of parameters
- Darius
- Printed and pasted the Grand master plan of our project on the wall
- Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
- Min Lin
- Send out enquiries for potential sponsors
- Waiting aproval for Proposal
- Hung
- Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
- Processing and storage of amplified DNA plasmids
- Updated Wiki website
- Choon Kit
- Inoculation and amplification of LacI-GFP and BBa_B0015 double terminator in LBA
- Processing and storage of amplified DNA plasmids
- Document laboratory reports for 02 Jun 2008
Lab details
- Hung,Choon Kit,Zhen Fu:
- Morning:
- Take out from the incubator 4 cryotubes. After spinning down, the tubes were observed:
- 2 tubes with LBA contained no cell pellets at all as expected.
- 2 tubes with LB without Amp. had cell pellets at the bottom. We then poured out the liquid and the tubes at -20 oC fridge for future use.
- Take out from the incubator 5 agar plates. All had cell colonies growing on them except for LBA BW-W3110 plate. We stored them at 4 oC fridge.
- Take out from the incubator 4 cryotubes. After spinning down, the tubes were observed:
- Afternoon:
- Inoculation to obtain 4 cryotubes:
- 2 containing LBA with BBa_B0015 plasmid cells
- 2 containing LBA with LacI-GFP plasmid cells
- Inoculation to obtain 4 cryotubes:
- Morning:
Incubate 4 tubes overnight
