Piotr:
1. Design of primers for sequencing something we want from pMPM-T5
Primers:
pMPMT5seqF pMPMT5seqR AIDseqP
Michał K.: 1. PCR - transcription fusion: AID + T7 RNA-polymerase</p>
AIDlNrH and T7pXbSal
Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion
Annealing temperature : Annealing time: 4 minutes
35 cycles