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Template:Team:UC Berkeley/Notebook/CDB notes
From 2008.igem.org
6/4/08 - Today we continued training and discussed the project.
6/6/08 - Jin streaked a plate with MC1061 E. coli cells and incubated at 37 C.
6/7/08 - Plate moved to fridge.
6/8/08 - I chose an isolated colony from the plate and inoculated a few mL of fresh LB media with it. The tube was incubated on a shaker at 37 C for 24 hrs.
6/9/08 - Took 200 uL of saturated culture and added it to 8 mL of LB media at 10:45 AM. Grew for 2 hrs on a shaker at 37 C.
Split culture into 3 tubes with 2 mL each. Culture 1 - Control (no sound), grew in foam box. Culture 2 - Music, high volume for 1 hr. Culture 3 - Ultrasound bath for 1 hr.
Note - Didn't have lysozyme thawed for RNA purification. Will repeat experiment tomorrow.
Inoculated a new tube of media with an isolated MC1061 colony.
