Team:Warsaw/Calendar-Main/20 May 2008

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Michał K:
1. PCR - translation fusion: AID + T7 RNA-polymerase - optymalization (temperature gradient 60 dgr;C - 80 dgr; C).

Primers:

AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion Annealing temperature: 55 °C Annealing time: 4 minutes 20 cycles 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase)



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