Team:Warsaw/Calendar-Main/2 June 2008

From 2008.igem.org

Revision as of 13:36, 1 October 2008 by MKrzyszton (Talk | contribs)

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Piotr:
1.Transformation of E.coli TOP10 with pZC320.

2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.

Michał K.:

PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase

Primers: AIDlNrH and T7pXbSal

Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)

Annealing temperature: 55°C

Time: 4 minutes

Number of cycles: 20