Michał K.:

1. Restriction digestion of pTrc99A-AID with HindIII and NcoI (1x Tango buffer).
2. DNA electrophoresis and isolation of DNA encoding AID from proper band (600 bp).
3. Restriction digestion of pMPM-T5 with HindIII and NcoI (1x Tango buffer), dephosphorylation (CIAP).
4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band (4250 bp).
5. Electrophoresis of isolated DNA
6. Ligation of isolated DNA fragments.