Team:Warsaw/Calendar-Main/19 May 2008
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Piotr:1. Gel electrophoresis (16 May PCR's) and DNA isolation from proper bands (600 bp for AID lane and 2700 bp for T7 polymerase lanes).
Michał L., Ewa, Marcin:Overnight inoculation and induction: Top10 + 0,5 mM IPTG pMPM-AID + Glc + 0,01% L-Ara pTrc99a-AID + Glc + 0,5 mM IPTG {{WarNotebookEnd}} |