Michał K:

1. PCR - translation fusion: AID + T7 RNA-polymerase - optimization (annealing temperature gradient 60°C - 80°C).

   Primers:

   AIDlNrH and T7pXbSal
   Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion
   Elongation time: 4 minutes
   35 cycles
   

2. Optimization of PCR - translation fusion: AID + T7 RNA-polymerase - MgCl₂ cocentration and number of cycles.

   Primers:

   AIDlNrH and T7pXbSal

   Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion
   Annealing temperature: 73°C
   Elongation time: 4 minutes
   
3. Gel electrophoresis of PCR products.

Michał L., Ewa, Marcin:

Plating colonies from the previous day on rifampicin