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Team:NTU-Singapore/Notebook/9 June 2008
From 2008.igem.org
Monday 9 June
- Hung,Zhen Fu:
- Prepare plasmid DNA(in liquid form) of :
- GFP producer x 2
- RBS 0032 x 2
- RBS 0034 x 2
- Prepare digestion solutions for 6 above plasmids for later gel electrophoresis.
- Prepare plasmid DNA(in liquid form) of :
- Choon Kit:
- Amplification of T7 promoter, 4 empty plasmids ([http://partsregistry.org/wiki/index.php?title=Part:pSB1A3 pSB1A3], [http://partsregistry.org/wiki/index.php?title=Part:pSB1AT3 pSB1AT3], [http://partsregistry.org/wiki/index.php?title=Part:pSB3K3 pSB3K3], [http://partsregistry.org/wiki/index.php?title=Part:pSB1AK3 pSB1AK3]) from the Registry (as gel electrophoresis was not successful last week).
- Gel electrophoresis running for GFP producer, RBS 0032 and RBS 0034.
- Chin Chong, Darius
- Prepare things to autoclave. (LB broth, pippette tips etc)
- Prepare lactose/IPTG solution and LacI-GFP containing E coli cells for LacI-GFP characterization
- Carry out trial for LacI-GFP characterization
