Preparation of constructs with OmpA protein fusions
Michał K.

1. Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).
2. PCR on isolated plasmids with [???]

PCR results
Michał L., Ewa, Marcin

We have successfully amplified Omega-linker, but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48°C. Mg²⁺ concentration elevated to 12 μM.