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Template:Team:UC Berkeley/Notebook/DV construction
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Contents |
K112701
K112702
Construction of <His-Tag! Basic Part K112702
Mixing of dv007/dv008 (27bp, EcoRI/BamHI/DpnI) Sub into (vector?)pBca1256 (EcoRI/BamHI, 2472+27, L) Product is pBca1256-K112110
dv007 Forward biobricking of <His-Tag! CATCATCATCATCATCATtaaGGATCC dv008 Reverse biobriquing of <His-Tag! GGATCCttaATGATGATGATGATGATG
PCR dv009/dv010 on pBca9145-Bca1117 (1060bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112100
dv009 Forward Biobricking of His-Tag> ccataGAATTCatgAGATCTatgCATCATCATCATCATCAT dv010 Reverse Biobricking of His-Tag> ATGATGATGATGATGATGcatAGATCTcatGAATTCtatgg
PCR bx1/bx2 on Bjh1301 (688 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112000
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bx1 Forward Biobricking of {T4 Holin!}
ccagtGAATTCatgAGATCTatggcagcacctagaatatc
bx2 Reverse Biobricking of {T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc
