Purdue/10 October 2008
From 2008.igem.org
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Dosed bacteria with 30 seconds of UV light (in the Bio-Rad hood). Covered (so impermeable to light) and put in 37C incubator for 1 hour.
Strangely, the bacteria already looked a little blue before UV exposure, but it could be a trick of the eyes. They were left out in the light of a window yesterday, but we're not sure if that would give them enough damage to go ahead and change colors...
After one hour of exposure, there is no significant difference between the UV-dosed bacteria and the non-dosed bacteria. We will wait to see if it just takes more time to develop color. Otherwise, we might expose them to larger amounts of UV radiation to see if that has an effect.
Edited by Janie Stine