1. Digest of pACYC177+OmpA_omega_deltaA_alpha (from 25 July) with BamHI and NotI. DNA ends blunting with Klenow fragment (3 hr).
2. Gel elctrophoresis and gel-out of proper band - 4300 bp.
3. Ligation of isolated DNA fragment (1 hr).
4. Transformationof E. coli TOP10 strain with ligation.
5. Transformants plating on LB + kanamycin.

Checking for presence of A on the cell membrane
Piotr

1. Pouring bacteria by drops onto nitrocellulose
2. Drying
3. Blocking
4. Anti-A antibody binding
5. Washing
6. Anti-rabbit antibody binding
7. Developing with BCIP and NBT