Team:Warsaw/Calendar-Main/23 June 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).

Preparation of alpa-A and omega-A fusions

Michał L., Ewa, Marcin

We have successfully amplified Omega-linker (Fig. 1), but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48°C. Mg2+ concentration elevated to 4 mM.

Fig. 1.PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.

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