Cloning of protein A DNA to pET15b+Omp-alfa plasmid

Antoni

1. Colony PCR on colonies from plates with transformations pGeneart+A Primers used: AP+NotI and AL+SacI
2. Lack of confirmed transformant colonies

Cloning of protein Z DNA to pET15b+Omp-alfa plasmid

Antoni

1. Digest pET15b+OmpA_alpha and pGeneart+A with NdeI and NotI, pET15b+OmpA_alpha dephosphorylation with CIAP
2. Gel electrophoresis of digested plasmids and gel-out of proper bands
3. Ligation of pET15b+alfa and A
4. Transformation of E. coli TOP10.
5. Transformants plating on LB + ampicillin.