Team:Warsaw/Calendar-Main/27 August 2008

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Cloning of protein Z DNA to pET15b+Omp-alfa plasmid

Antoni

Transformant colonies inoculated to liquid LB with ampicillin.

Cloning of protein A DNA to pET15b+Omp-alfa plasmid

Michał K.

  1. Digestion of pGeneart+A and pET15b+Omp-alfa with NdeI and NotI (Orange buffer), pET15b was also dephosphorylated.

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