Preparation of constructs with OmpA protein fusions

Michał K.

Repetition of PCRs and gel-out purifications from 16 June.

PCR of Alpha-link and link-A

Michał L. Marcin and Ewa

1. We've run gradient PCR to obtain Alpha-link and link-A.
   Annealing temp 48°C - 55°C.
   Elongation time 1 - 2 minutes.
2. We have successfully amplified Alpha-link and link-A. Now it's time for PCL (Polymerase Chain Ligation) to create fusions Alpha-A and Omega-A (Fig. 1).

Change of the reporter from pZC with B-galactosidaze to GFP or RFP

Piotr, Weronika

1. Inoculation 10ml LB with E.coli TOP10 carrying pZC
2. Isolation and chemotransformation with plasmids nazwa (Gfp genetrator), nazwa (RFP generator)