"
Team:University of Lethbridge/Notebook/GeneralLabJune
From 2008.igem.org
Contents |
June 6 2008
Sebastian and Roxanne
Prepared 1L of semi-solid media following the procedure found on OpenWetWare.
-10g peptone -10g Agar -10g NaCl -5g Yeast Extract
Semi-solid Media was autoclaved and stored in the iGEM fridge for making plates at a later date.
June 10 2008
Christa, Munima, Roxanne, and Sebastian
Prepared 1L of liquid media following the procedure found on OpenWetWare.
-10g peptone -10g NaCl -5g Yeast Extract
36 culture test tubes were poured, and the remainder was left in a 1L Erlenmeyer flask. Both were autoclaved and stored in the iGEM fridge.
Christa
Took and inventory of iGEM 2007 parts in Wieden -80 freezer
Roxanne
Defrosted the -20 freezer in the teaching lab for iGEM use (with a little help from my friends!)
June 11 2008
Sebastian, Munima, Roxanne, Christa
Poured 8 minimal media (labeled control- with blue sharpie) plates and 14 ampicillin plates (labeled ampicillin- with red sharpie)with aseptic technique and stored in the 4C fridge. Ampicillin concentration is 50ug/mL according to the Topp and Gallivan paper.
