Team:MIT

Welcome to the MIT team Wiki for iGEM 2008 iGEM is the international Genetically Engineered Machines competition. The objective of the competition is to design and build an engineered biological system using [http://en.wikipedia.org/wiki/DNA DNA]. To see examples of the amazing possibilities of iGEM, check out last years [http://parts.mit.edu/igem07/index.php/Main_Page iGEM page] Read our promotional brochure to learn about synthetic biology at MIT (front and back). iGEM at MIT is possible because of outside support, contact Tom Knight (tk [at] mit.edu) to help out! iGEM at MIT is partly supported by the [http://web.mit.edu/urop/basicinfo/ Undergraduate Research Opportunity Program] and faculty including Drew Endy and Tom Knight. We guarantee UROP funding for undergrads. We need help to continue supporting undergrads, paying registration fees, and supplying lab reagents. Read more about our team on our fundraising notebook

Biogurt: A Sustainable and Savory Drug Delivery System

Streptococcus mutans, the main cause of dental caries, binds to glycoproteins on the teeth. A clinical study (Kelly CG et al.; Nature Biotechnol. 1999) isolated the 20aa functional segment (p1025) that S.mutans uses to attach to the teeth. p1025 competitively inhibits the binding of S.mutans, causing unharmful bacteria to grow in its place, preventing the recolonization of S.mutans for 90 days.

We are engineering Lactobacillus bulgaricus, a bacteria commonly found in yogurt, to produce and secrete this peptide under a promoter activated by lactose.

The peptide p1025 could simply be added to any food. But production of this peptide by L. bulgaricus is an independent process, so inserting the gene into live bacteria in yogurt will enable continuous production. Since a new batch of yogurt can be made using the bacteria from a small amount of the old batch, a continuous supply of teeth-cleaning yogurt will be available from the first successfully engineered batch. This could be the key to providing effective dental health care in underdeveloped rural communities, especially if yogurt is already an integral part of the diet.

Also, the p1025 gene could be replaced by any other gene, so this same expression system could be used to produce other useful peptides. Yogurt with modified bacteria will provide a cheap, efficient, and delicious way to distribute vitamins, vaccines and more.