Repeated the successful PCR reaction from before.
This time we obtained successful band for both srlD 700bp and the srl operon 2700bp
These products were purified and stored at 4C overnight.
Some of the product was used to start a second PCR reaction to run overnight.
Gowth curves using revised Cmedia with additional components completed to determine optimal growth media.
Team Fungus:
Grew BL21 pET28a plates for colonies 1 & 2, streaked out new plates, and selected colonies and grew them in liquid media over the weekend.
Minipreped to purify DNA from colonies and stored them on ice.
Ran a second PCR to test results and get more colonies, however electrophoresis returned inconclusive results.
Digested purified plasmid and ran out on 1% agarose gel. Colonies 1a and 1b possibly have insert.
Ran PCR using previous PCR products as template. Ran out on 1% agarose gel. Saw faint bands.
Ran a PCR using purified plasmids as templates.
Made LB broth.
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