NTU@iGEM Project Introduction Methodology Team Introduction Team background Notebook Dry Lab Introduction ODEs used Modeling Constructs Parameters used Deterministic Modeling Sensitivity Analysis Stochastic Modeling Wetlab Introduction Materials and Equipment Protocols Verification of Detection & Lysis System Our New Parts Characterization Introduction Standard Promoters Characterization of pLacI-GFP pLacI-GFP images and clips Characterization of pLsrA-YFP New characterization Proposal Parameter Estimation & Correlations Acknowledgements

Contents 1 Introduction 1.1 Abstraction 1.2 Decoupling 1.3 Standardization

Introduction

We employed the methodology used on basic engineering concepts: Abstraction, decoupling and standardization.

Abstraction

In abstraction we reduce the complexity of things by creating simple parts and developing these parts into higher complex devices and systems

Decoupling

In decoupling, we decoupled each individual part from the others, devising them without consideration for the other existing part. Following which the fusion of different combination of components formed a working device

Standardization

in standardization, we aim to establish standards that are widely used and agreed. We also allowed the predicament of components and compatibility of parts leading to a set of standards and protocols that could be used for future teams.