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Materials and Methods
From 2008.igem.org
Materials and Methods
1. Cloning
Constructs were cloned using the AarI method, developed by Sergio Pesajovich for the 2007 iGEM competition. To learn more about AarI cloning:
Everything you ever wanted to know about AarI
In brief: yeast genomic DNA was used as a template to PCR amplify "parts". Parts were TOPO cloned, validated by sequencing, and then combinatorially cloned into acceptor vectors:
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