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HOME	PROJECT	TEAM	SUBMITTED PARTS	MODELING	WETLAB	LAB-BOOK	BIOSAFETY AND PROTOCOLS

Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.

1 Protocols
2 Week 1: from 07/21/08 to 07/27/08
3 Week 2: from 07/28/08 to 08/03/08
4 Week 3: from 08/04/08 to 08/10/08
5 Week 4: from 08/11/08 to 08/17/08
6 Week 5: from 08/18/08 to 08/24/08
7 Week 6: from 08/25/08 to 08/31/08
8 Week 7: from 09/01/08 to 09/07/08
9 Week 8: from 09/08/08 to 09/14/08
10 Week 9: from 09/15/08 to 09/21/08
11 Week 10: from 09/22/08 to 09/28/08
12 Week 11: from 09/29/08 to 10/05/08
13 Week 12: from 10/06/08 to 10/12/08
14 Week 13: from 10/13/08 to 10/19/08
15 Week 14: from 10/20/08 to 10/26/08
16 Week 15: from 10/27/08 to 10/29/08

Protocols

For more information, see Biosafety page

Protocol	Biosafety level
Plates preparation	1
Amplification	1
Transformation	1
Inoculation	1
Miniprep	1
Digestion reaction	1
Gel preparation	2
Electrophoretic run	2
Gel extraction	2
Ligation reaction	1
Chemiocompetent cells	1
Mediums and buffers	1
Antibiotics stocks preparation	1
IPTG stocks preparation	1
Fluorescence test	1
M9 supplemented media	1

Week 1: from 07/21/08 to 07/27/08

General Preparations

Preparation of chemiocompetent cells from E. Coli DH5α, Top10 and DB 3.1
Preparation of antibiotic stocks for Ampicillin and Kanamicin
Preparation of LB medium and LB plates for cloning.

Week 2: from 07/28/08 to 08/03/08

1. Eluition and Amplification from 2008 Registry Collection: [http://partsregistry.org/Part:BBa_R0082 R0082,] [http://partsregistry.org/Part:BBa_R0083, R0083], [http://partsregistry.org/wiki/index.php/Part:BBa_M30109 M30109] in TOP10 strain to build and characterize the Light response system to be our spatial selective trigger.

2. Eluition and Amplification from 2008 Registry Collection: [http://partsregistry.org/Part:BBa_E0240 E0240], [http://partsregistry.org/Part:BBa_P1010 pSB3K3_P1010]in DB3.1 and the Practice Promoter Set ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J23103/ J23150, J23151, J23102]) to test and set up the new [http://partsregistry.org/Measurement Biobrick Standard Measurement Protocol]

Week 3: from 08/04/08 to 08/10/08

Week 4: from 08/11/08 to 08/17/08

Week 5: from 08/18/08 to 08/24/08

Week 6: from 08/25/08 to 08/31/08

Week 7: from 09/01/08 to 09/07/08

Week 8: from 09/08/08 to 09/14/08

Week 9: from 09/15/08 to 09/21/08

Week 10: from 09/22/08 to 09/28/08

Week 11: from 09/29/08 to 10/05/08

Week 12: from 10/06/08 to 10/12/08

Week 13: from 10/13/08 to 10/19/08

Week 14: from 10/20/08 to 10/26/08

Week 15: from 10/27/08 to 10/29/08

Team:Bologna/Notebook