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Team:UCSF/Willis Wong Notebook
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Contents |
Willis Wong
Notebook
Overall Objective
Devise an array of inputs that can be linked to regulation of silencing.
Milestones
1. Define minimal fragment for light inducible dimerization.
2. Define the configuration of dimerization/regulatory domains that will yield desired function. (Silencing/Unsilencing)
Work Done
Week 1-2: Design AB and BD parts for combinatorial cloning (includes PhyB and Pif3 parts). Week 3-4: Ligate AB + BD parts into 315 vectors. Week 4-5: Subclone Pif3 constructs into 303 vectors for intregration into a yeast reporter strain [B3(H)]. Week 7-9: Transform the PhyB constructs into the yeast strains that were integrated with Pif3. Week 10-11: In the dark, added PCB, pulsed with respective light, ran FACs.
