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From 2008.igem.org

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13 September 2008 <|> 15 September 2008

Laboratory work

Team:Communication

(13/9)--->Mini prep

Transformation

Competent cells : XL10G

• BBa_T9002[http://partsregistry.org/Part:BBa_T9002](2007)
• Plac+RhlI+LVA
• Plac+CinI+LVA

--->(15/9)

Team:Output

Time Responce Liquid
Digestion test

• [http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
• [http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
• [http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
• [http://partsregistry.org/Part:BBa_C0070 BBa_C0070]
• [http://partsregistry.org/Part:BBa_J06620 BBa_J06620]
• [http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
• [http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
• BBa_I716542
• [http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
• [http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
• [http://partsregistry.org/Part:BBa_J01101 BBa_J01101]
• [http://partsregistry.org/Part:BBa_C0071 BBa_C0071]
• [http://partsregistry.org/Part:BBa_I712019 BBa_I712019]
• [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
• [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
• [http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
• [http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
• [http://partsregistry.org/Part:BBa_I7100 BBa_I7100](x2)
• [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]

Sample No	each
DNA(digested sample)	3
Buffer2(x10)	1
dH2O	6
EcoRⅠ	0.075
TOTAL	10

-->37℃ 1hour

Gel check

Sample No	each
DNA(digestion sample)	10
Loading Dye	2
TOTAL	12

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