Team:Warsaw/Calendar-Main/30 September 2008

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Mutagenesis of protein A

Paweł

Results of sequencing: unfortunately all sequences where wild-type.

Preparation of BioBricks

Michał K.

  1. Isolation of HQs(??????) plasmids.
  2. Digest of isolated plasmids with EcoRI and BcuI (BamHI buffer).
  3. Digest of pACYC177+OmpA_omega_deltaA_alpha with BamHI and BglII (BamHI buffer).
  4. Gel elctrophoresis and gel-out of proper band: ??????.
  5. Gel elctrophoresis. Chioce of proper clone.


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