NTU@iGEM Project Introduction Methodology Team Introduction Team background Notebook Dry Lab Introduction ODEs used Modeling Constructs Parameters used Deterministic Modeling Sensitivity Analysis Stochastic Modeling Wetlab Introduction Materials and Equipment Protocols Verification of Detection & Lysis System Our New Parts Characterization Introduction Standard Promoters Characterization of pLacI-GFP pLacI-GFP images and clips Characterization of pLsrA-YFP New characterization Proposal Parameter Estimation & Correlations Acknowledgements

Contents 1 Parameter k3DE analysis 1.1 k3DE variation 1.1.1 k3DE = 0.00000001 1.1.2 k3DE = 0.0000001 1.1.3 k3DE = 0.000001 1.1.4 k3DE = 0.00001 1.1.5 k3DE = 0.0001 1.1.6 k3DE = 0.001 1.1.7 k3DE = 0.01

Parameter k3DE analysis

In the graphs below, the blue lines represent the lysis protein production under iron induction alone. The red lines represent the production of lysis protein when no iron or Auto-inducer 2 is added.

k3DE variation

k3DE = 0.00000001

k3DE = 0.0000001

k3DE = 0.000001

k3DE = 0.00001

k3DE = 0.0001

k3DE = 0.001

k3DE = 0.01

It is clear that when k3DE = 0.00000001 will there be a pronounced difference between the different situations. It is the main reason why this parameter was chosen to take on this value even though it is not very realistic.