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From 2008.igem.org

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13 September 2008 <|> 15 September 2008

Laboratory work

Team:Communication

(13/9)--->Mini prep

Transformation

Competent cells : XL10Gold

• [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)
• [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084009 BBa_K084009](Plac+RhlI+LVA)
• [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA)

--->(15/9)

Team:Output

Time Responce Liquid

Digestion test

• [http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
• [http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
• [http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
• [http://partsregistry.org/Part:BBa_C0070 BBa_C0070]
• [http://partsregistry.org/Part:BBa_J06620 BBa_J06620]
• [http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
• [http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
• BBa_I716542
• [http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
• [http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
• [http://partsregistry.org/Part:BBa_J01101 BBa_J01101]
• [http://partsregistry.org/Part:BBa_C0071 BBa_C0071]
• [http://partsregistry.org/Part:BBa_I712019 BBa_I712019]
• [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
• [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
• [http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
• [http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
• [http://partsregistry.org/Part:BBa_I7100 BBa_I7100](x2)
• [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]

Sample No.	each
DNA(digested sample)(μL)	3
Buffer2(x10)(μL)	1
dH2O(μL)	6
EcoRⅠ(μL)	0.075
TOTAL(μL)	10

--> left at37 degrees for 1 hour

Gel check

Sample No.	each
DNA(digestion sample)(μL)	10
Loading Dye(μL)	2
TOTAL(μL)	12

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