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Team:Chiba/Calendar-Home/16 September 2008
From 2008.igem.org
15 September 2008 <|> 17 September 2008
Laboratory work
Team:Communication
(15/9)-> time-delay test
- Transformed these plasmids into E.coli strain JW1908.(also transformed [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] into E.Coli strain JW1908.)
- [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
- [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
- [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
- [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
- conditions
- temparature
37℃ and 30℃
Team:Output
Digestion test
- [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)(x2)
- [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)(x5)
- [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)+[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)(x2)
- [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)
- [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)
- [http://partsregistry.org/Part:BBa_S03160 BBa_S03160](RBS+cinR)
| Sample No. | each |
| DNA(μL) | 3 |
| EcoRⅠ(μL) | 0.083 |
| PstⅠ(μL) | 0.083 |
| BSA(x10)(μL) | 1 |
| Buffer3(x10)(μL) | 1 |
| dH2O(μL) | 5 |
| TOTAL(μL) | 10 |
-->37℃ 1hour
| Sample No. | each |
| DNA(μL) | 10 |
| Loading Dye(μL) | 2 |
| TOTAL(μL) | 12 |
- [http://partsregistry.org/Part:BBa_J37019 BBa_J37019](LuxI+RBS+LuxR)->no colony
- [http://partsregistry.org/Part:BBa_J37033 BBa_J37033](RBS+LuxR)
- [http://partsregistry.org/Part:BBa_J37020 BBa_J37020](LuxI+LuxR+pLux+GFP)
- [http://partsregistry.org/Part:BBa_J37032 BBa_J37032](pLux+GFP)
- Venus YFP(ptet+LuxR+YFP)
| Sample No. | YFP |
| DNA(μL) | 1 |
| Venus Fwd(μL) | 5 |
| Rev(VR)(μL) | 5 |
| Thermo pol Buffer(μL) | 10 |
| dNTP mix(μL) | 10 |
| Vent(μL) | 1 |
| dH2O(μL) | 68 |
| TOTAL(μL) | 100 |
