Team:Warsaw/Calendar-Main/10 October 2008

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Visit in US Embassy

Visas have been accorded to the whole team.

Preparation of BioBricks

Michał K.

  1. Digest of RFP(?????)+deltaA with EcoRI and SacI (BamHI buffer), dephosphorylation with CIAP
  2. Gel electrophoresis and gel-out of proper band 2200 bp.
  3. Ligation of digested 5kb (??????) vector from (30 September) with pT7_omega_link and RFP(?????) with pT7_alpha fragment (1 hr).

    4. Piotr

      Piotr

      1. Transformation of TOP10 with above ligations.
      2. Tranformants planting on LB with kanamycin.


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