Tests for ampicillin resistance given by protein added to medium

Piotr

Cloning of protein A DNA to pET15b+OmpA-alpha plasmid

Michał K.

1. Digest of pGeneart+A and pET15b+OmpA-alpha with NdeI and NotI (Orange buffer), pET15b was also dephosphorylated.
2. Gel electrophoresis of digested plasmids (Fig. 1) and gel-out of proper bands (400 bp for A lane and 6200 bp for pET15b lane).
3. Ligation of pET15b-Alpha and A.
4. Transformation of E. coli TOP10.
5. Transformants plating on LB + ampicillin.
Fig. 1. Control NdeI/NotI digest of isolated plasmid pGeneart+A
1. Marker
2. digested plasmid pGeneart+A