Change of the reporter from pZC320 with B-galactosidase to GFP or RFP

Piotr, Weronika

1. There were blue and white colonies but no red or green fluorescence in UV
2. Inoculation of liquid LB medium + Amp with white colonies

Preparation of constructs on pET15b: OmpA_alpha and OmpA_omega

Paweł

Colonies of each transformants from previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) were inoculated to liquid LB + ampicillin.

Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin

We have received sequencing results: A is OK but in case of Alpha-A and Omega-A the result is weird. It seems that there were error in synthesis of OmegaP+link10+homo2 and AlphaP+link10+homo2 primer which causes frameshift and cloning of these fusions have to be repeated from the beginning.