Preparation of pSB2K3 + linker_alpha (BBa_K103009)

Michał K.

1. Isolation of plasmids from culture inoculated on previous day.
2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Preparation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016)

Michał K.

Inoculation of colonies from plate with new ligation of pACYC177 + OmpA-linker-omega-linker (BBa_K103016) to LB with kanamycin.

Preparation of pT7_alpha_link

Michał K.

1. Control digest of isolated plasmid - pSB1A3 carrying pT7_alpha fragment (14 October) with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.
2. Overnight digest pSB1A3 carrying pT7_alpha with EcoRI and BcuI (BamHI buffer).

Preparation of BioBricks

Michał K.

1. Isolation of plasmids from culture inoculated on previous day 3kb(?????)+ link_alpha, 3kb(????)_pLac_OmpA_ + alpha2 and RFP(?????) + AraC+pBAD+AID.
2. Control digest of isolated plasmids: 3kb(?????)+ link_alpha, 3kb(????)_pLac_OmpA_ + alpha2, RFP(?????) + AraC+pBAD+AID and RFP(?????) + pT7_alpha (14 October) with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found for RFP(?????) + AraC+pBAD+AID ligation but we found good clones for other ligations.
3. Repetition of overnight ligation of isolated DNA fragments: RFP(?????) + AraC+pBAD+AID.