Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

1. Isolation of plasmids from cultures inoculated on previous day - pSB1A3 + AraC+pBAD+AID.
2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found. We didn't manage to obtain this BioBrick.

Preparation of OmpA-linker-alpha-linker

Michał K.

1. Isolation of plasmids from cultures inoculated on previous day - OmpA_alpha_.
2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr

1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.