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Team:Hawaii/Notebook/2008-06-19
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Alkaline Lysis Miniprep
- Krystle & Margaret
- Plasmid prep was performed according to the experimental protocol
- Plasmid prep was performed with:
| part number | antibx media strength | source organism |
|---|---|---|
| E0040 (GFP) | amp100 | DH5&alpha |
| J04430 (lac+GFP) | DH5&alpha | |
| B0034 (RBS) | GP's super competent cells | |
| B0024 (TT) | DH5&alpha |
| type | antibx media strength | source organism |
|---|---|---|
| pUC18 | amp100 | DH5&alpha |
| pBLUSCRIPT | ||
| RP1 | kan50 | E. coli |
Cryo-Stocks
- Krystle
- Made cryo-stocks of pBLUSCRIPT, pUC18, and B0024.
Run Gel for pRL1383a plasmid Prep
- Norman
- See experiment for details.
Drylab Work
Primer Discussion
- Krystle, Norman, & Margaret
- Discussed the format of primers
- worked on revising primers
Discussion
Quote of the Day
Note to self: if I have weird cancers in strange places, its probably because I was playing with these carcinogens. - KS
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
