Team:Edinburgh/Results

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Revision as of 22:39, 27 October 2008 by Adlerma (Talk | contribs)

Results and current status as of 29 October 2008. We are hoping to obtain some last-minute results to present at the Jamboree.

Contents

Overview

Our results are promising but our dream of using bacteria to convert cellulose into starch and β-carotene is still unrealised. Fortunately, the work will be continued by two undergraduate students after Christmas.

Cellulolysis device

Edinburgh Map1 Cellulolysis.jpg

  • cenA (endoglucanase) and cex (exoglucanase): Biobricks made, but encountered problems when adding ribosome binding site.
  • bglX (beta glucosidase): Biobrick made.
  • PcstA (promoter): Biobrick made and tested - successful.

Glycogenesis device

Edinburgh Map2 Glycogen.jpg

Starch synthesis device

Edinburgh Map3 Starch.jpg

  • su1 and iso2 (isoamylases): Work in progress - to be continued in an undergraduate honours project.

Lycopene generator

Edinburgh Map4 BCarotene.jpg

  • dxs+crtE+crtB+crtI+crtY: Work in progress - encountered problem at final step.

Limonene synthase device

Edinburgh Map5 Limonene.jpg

  • dxs+LIMS+appY: Biobrick made - still being tested (last test failed to detect limonene synthesis).

Acknowledgements

We would like to thank the following researchers for their contributions to our project:

  • Raman spectroscopy for glycogen production was done by Dr Rabah Mouras.
  • Mutagenesis of crtI was done by Douglas Armstrong.
  • The maize isoamylase genes su1 and iso2 were generously donated by Professor Alan Myers of Iowa State University.