Team:Warsaw/Calendar-Main/22 October 2008

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Preparation of AID under pBAD/araC (BBa_K103002)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day - pSB1A3 + AID under pBAD/araC (BBa_K103002).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found. We didn't manage to obtain this BioBrick.

Preparation of OmpA_linker_alpha_linker (BBa_K103014)

Michał K.

  1. Isolation of plasmids from cultures inoculated on previous day - pSB2K3 + OmpA_linker_alpha_linker (BBa_K103014).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.
Fig.1.Control EcoRI/PstI digests of isolated plasmids. 1. Marker
2-9. EcoRI/PstI digests of isolated plasmids

Preparation of OmpA-linker-omega-linker (BBa_K103016)

Piotr

  1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
  2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - proper clones found.

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